Evaluation of relative efficiency of oocyte collection techniques from abattoir-derived goat ovaries

Abstract

Background: This study aimed to compare different parameters between right and left ovaries, ovaries with corpus luteum, and without corpus luteum, and to determine the most effective oocyte collection technique.

Methods: The experiment was conducted at the Department of Animal Nutrition, Genetics, and Breeding at Sher-e-Bangla Agricultural University, Dhaka- 1207, from January 2019 to December 2019. A total of 431 ovaries were collected from different abattoirs in Dhaka. Oocytes were harvested through different oocyte collection techniques like aspiration, slicing, and puncture. Data on parameters like weight, length, width, total number of visible follicles, number of follicles aspirated, collected grade A, grade B, grade C, and grade D cumulus-oocyte- complexes (COCs) per ovary, total number of COCS were recorded following standard procedures.

Results: The length (cm) of the right ovaries (1.31±0.04) was found to be significantly (p<0.05) greater than that of the left ovaries (1.18±0.04). The number of normal COCs (Grade A and Grade B) was significantly (p<0.01) higher in left ovaries (2.14±0.08 and 1.65±0.08) compared to right ovaries (0.36±0.08 and 0.23±0.08), respectively. Additionally, ovaries without a corpus luteum (CL) had a significantly (p<0.01) higher number of normal COCs (Grade A and Grade B) (1.21±0.07 and 0.90±0.07) compared to those with a CL (0.32±0.09 and 0.29±0.09), respectively. The number of normal COCs (Grade A and Grade B) was significantly (p<0.05) higher using aspiration (3.80±0.60 and 3.33±0.42) compared to slicing (3.67±0.78 and 3.20±0.54) and puncture (2.20±0.60 and 1.40±0.42), respectively.

Conclusion: The highest yield of normal COCs was obtained through aspiration, followed by slicing and puncture. Left ovaries contained more normal COCs and a higher number of follicles compared to right ovaries. Additionally, ovaries without a corpus luteum had a higher number of follicles and normal COCs than those with a corpus luteum. Therefore, it can be concluded that left ovaries, ovaries without a corpus luteum, and the aspiration technique are optimal for obtaining quality COCs.