Seroprevalence and antibiotic sensitivity of Salmonella spp. in commercial layer chicken of Pirojpur district

S. Rahman; M. A. Rahman; M. S. Ahmed

S. Rahman Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal-8210, Bangladesh
M. A. Rahman Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal-8210, Bangladesh
M. S. Ahmed Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal-8210, Bangladesh

Keywords: Multidrug resistance, age, flock size, risk factors

Abstract

Background: Salmonellosis is one of the most important diseases of poultry that seriously impedes the development of the poultry industry. The study was investigated to determine the seroprevalence and antibiotic sensitivity of Salmonella spp. in commercial layer chicken at study area.

Methods: The study was conducted from June 2020 to July 2020. For seroprevalence study, a total of 200 serum samples were randomly collected from 20 layer farms at Nesarabad Upazila of Pirojpur district, Bangladesh. The Salmonella spp. were isolated and identified by conventional methods like culture, Gram’s staining and biochemical test. The risk factors for salmonellosis in layer birds were identified by multivariable logistic regression analysis.

Results: The overall seroprevalence of salmonellosis in layer chicken was 58% (95% confidence interval [CI]: 50.8; 64.8). The seroprevalence was significantly higher (71.25%) in > 50 weeks age (Odd ratio [OR]=4.5; 95% CI: 1.94; 10.3) than that of > 15-30 weeks age (37.5%). In addition, the seroprevalence of salmonella infection was also significantly higher (74%) in medium (OR=2.3 ;95% CI: 1.1; 4.7) and large flocks (OR=4.7; 95% CI: 1.9; 11.3) compared to small flocks. The Salmonella spp. was found to be distributed in 68.75% liver, 25% spleen, and 18.75% intestinal swab. Antibiogram study showed that 63.64% of Salmonella spp. was multidrug resistance (MDR). Salmonella isolates were most resistant to oxytetracycline (90.91%) followed by amoxicillin (81.82%) and sulfamethoxazole-trimethoprim (63.64%); but the isolates were most sensitive to ceftriaxone (100%) followed by gentamicin (81.82%), ciprofloxacin (72.73%) and streptomycin (63.64%).

Conclusions: The seroprevalence of salmonellosis in the layer bird of the study area seems to be very high. Aged layer birds of medium and large flocks should be targeted for future control and surveillance program. Antibiogram guided treatment may help prevent treatment failure and development of antimicrobial resistance

DOI: https://doi.org/10.33109/bjvmjd2022am1

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Author Biographies

S. Rahman, Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal-8210, Bangladesh

M. A. Rahman, Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal-8210, Bangladesh

M. S. Ahmed, Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barisal-8210, Bangladesh